Molecular characterization of VIM1 isolates of Pseudomonas aeruginosa isolated from cases of sinusitis
نویسندگان
چکیده
Methods This study describes the molecular characterization of 15 Pseudomonas aeruginosa strains, isolated from endoscopic pus specimens, obtained from sinusitis cases. Susceptibility testing was performed by disk diffusion assay. The MIC of ceftazidime was determined by E test. Ceftazidime (CDM) EDTA combined disc test was used to screen for class B b lactamase production. Plasmid DNA extraction was performed by alkali lysis method. The carriage of the MBL gene, blaVIM and IMP1 gene were screened by PCR. PCR products of 7 positive strains for MBL were sequenced and analysed using bioinformatics tools.
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عنوان ژورنال:
دوره 14 شماره
صفحات -
تاریخ انتشار 2014